Wound Multiplex PCR Reagents
with AMR markers

Wound Infection (WN) multiplex PCR assays are intended for the qualitative detection of pathogens and associated Antimicrobial Resistance (AMR) markers. The Scienetix WNI multiplex PCR reagent kit contains multiplex PCR assays for the detection of bacteria (22) and fungi (6), along with associated AMR (18) markers and internal control. The Scienetix multiplex reaction mixes (WRM-1 to WRM-16) are configured as open system reagents (OSR) for use with any real-time PCR system. Fine-tuned multiplex reactions are available in 2ml tubes or pre-plated 384 well plates.

The Scienetix Wound kit consists of pre-formulated multiplex PCR reactions and standards for monitoring assay performance.

  • WNI PCR Reaction Mix (WNRM-1 to 16)
  • Human Internal control (HIC)
  • Amplification Control (AC)
Bacteria Fungi AMR
Enterococcus faecalis Candida albicans Vancomycin Resistance (VanM)
Enterococcus faecium Candida tropicalis Carbapenem Resistance (NDM)
Enterobacter cloacae Candida parapsilosis Carbapenem Resistance (KPC)
agalactiae Candida glabrata Beta Lactamase Resistance (TEM)
Streptococcus pyogenes Epidermophyton floccosum Carbapenem Resistance (OXA)
Klebsiella aerogenes Trichophyton rubrum Tetracycline Resistance (TetM)
Proteus mirabilis Aminoglycoside Resistance (ANT-la)
Klebsiella pneumoniae Beta Lactamase Resistance (SHV)
Pseudomonas aeruginosa Aminoglycoside Resistance (APH 3)
Staphylococcus aureus Fluoroquinolone Resistance (GyrA)
Morganella morganii Fluoroquinolone Resistance (qnr)
Klebsiella oxytoca Tetracycline Resistance (tetB)
Proteus vulgaris Methicillin Resistance (mecA)
Aerococcus urinea Vancomycin Resistance (vanA)
Escherichia coli Sulfonamide Resistance (sul1)
Actinotignum schaalii Trimethoprim Resistance (drfA1)
Citrobacter freundii/braakii/koseri VanB Resistance (vanB)
Acinetobacter baumannii Beta Lactamase (CTX-M-Grp1)
Serratia marcescens
<>Prevotella bivia
Staphylococcus saprophyticus
Bacteroides fragilis

Instruction for Use

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Safety Data Sheet

Sensitivity: < 10copies/ul
Run Time: Approx 1:00 hr (depending on instruments)
Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time PCR platforms.
Catalog# Reactions
WND48-96 96 Wound Multiplex PCR Reagent mix with AMR markers- 96 reactions
WND48-384 384 Wound Multiplex PCR Reagent mix with AMR markers- 384 reactions
WND48-384PT 24 Wound Multiplex PCR Reagent mix with AMR markers- Pre-filled 384 well plate - 24 reactions

For Research Use Only

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