Ear, Nose & Throat (ENT) infection
with AMR Markers

This ear, nose, and throat (ENT) polymerase chain reaction (PCR) panel is intended for qualitatively, or semi-quantitatively, detecting ENT infection microorganism and antibiotic-resistant genes associated with the pathogens. This Scienetix molecular reagent ENT kit contains multiplex qPCR assays for detecting thirty-two (32) bacteria and six (6) fungal pathogens. The Scienetix kit also includes eighteen (8) assays for detecting antibiotic resistance genes and one (1) internal control. This ENT kit includes twelve (12) validated multiplex reaction mixes (ERM-1 to ERM-12) and all associated qPCR assay controls. The ENT qPCR reagents are manufactured and packaged as an open system reagent (OSR) for use with open system platforms. Examples of open system platforms are the Applied Biosystems QuantStudio (Design & Analysis software version 1.5.1 or later), Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time qPCR platforms.

This ENT reagent kit consists of pre-formulated qPCR reactions and standards for monitoring the assay performance. Scienetix reagents are available in a pre-mixed reaction mix provided in 2mL tubes or pre-plated in 96 and 384 well plates. It is recommended to test amplification controls (AC) and negative controls (NC) with each lot (qPCR plate) of sample testing.

ENT PCR Reaction Mix Tubes (ERM-1 to 12) -750µl 12 -20°C
Amplification Control (AC) – 200µL 1 -20°C
Bacteria(32) Fungi(6) AMR(8)
Bacteroides fragilis Candida albicans CTX-M
Hemophilus influenzae Candida auris NDM
Alloiococcus otitis Aspergillus sp KPC
Aeromonas sp Candida galbrata IMP
Enterobacter cloacae Candida tropicalis VanB
Citrobacter freundii /koseri/braakii Candida parapsilosis OXA-48
Corynebacterium urealyticum toxin VIM
Corynebacterium amycolatum VanA
Acinetobacter baumannii
Enterococcus faecalis
Enterococcus faecium
Klebsiella aerogenes
Moraxella catarrhalis
Klebsiella pneumoniae
Klebsiella oxytoca
Prevotella bivia
Moraxella lacunata
Propionibacterium acnes/Cutibacterium acnes
Peptostreptococcus sp
Serratia marcescens
Pseudomonas aeruginosa
Staphylococcus epidermidis
Staphylococcus aureus
Staphylococcus Saprophyticus
Staphylococcus haemolyticus
Stenotrophomonas maltophilia
Staphylococcus lugdunensis
Vibrio sp/Vibrio vulnificus
Streptococcus pneumoniae
Stretococcus pyogenes
Escherichia coli
Streptococcus agalactiae (Group B)

Instruction for Use

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Safety Data Sheet

Sensitivity: < 10copies/ul
Run Time: Approx 1:00 hr (depending on the instrument)
Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time PCR platforms.
Catalog# Reactions
Quantity per Kit Description
ENT48-96PT 96 12 ENT Multiplex qPCR Reagents pre-plated 96 well plates (8 samples per plate)
ENT48-384PT 96 3 ENT Multiplex qPCR Reagents pre-plated 384 well plates (32 samples per plate)
ENT48-96 96 1 ENT Multiplex qPCR Reagents pre-mixed vials ERM 1-12

For Research Use Only

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