Ear Nose and Throat (ENT) Pathogen
PCR Reagent Kit

This ENT pathogen polymerase chain reaction (PCR) reagent kit is intended for qualitative, or semi-quantitative, detection of ENT pathogens and the antibiotic-resistant genes associated with the pathogens. This kit includes twelve (12) pre-formulated PCR reactions along with one (1) positive assay control and one (1) exogenous extraction control (EEC) for monitoring the assay performance. Reagents are available in pre-mixed reactions in 2 mL tubes or pre-plated in 96 and 384 well plates.

This RPP qPCR reagent kit consists of pre-formulated qPCR reactions and standards for monitoring assay performance.

  • PCR Reaction Mix
  • Positive Control (PC)
  • Exogenous Extraction Control (EEC)
Bacteria Fungus AMR
Acinetobacter baumannii Aspergillus spp Beta Lactamase Resistance (CTX-M-Grp1)
Aeromonas spp Candida albicans Beta Lactamase Resistance (IMP)
Alloiococcus otitis Candida auris Beta Lactamase Resistance (VIM)
Bacteroides fragilis Candida glabrata Carbapenem Resistance (KPC)
Citrobacter freundii/ braakii/koseri Candida parapsilosis Carbapenem Resistance (NDM)
Corynebacterium amycolatum Candida tropicalis Carbapenem Resistance (OXA-48)
Corynebacterium urealyticum Vancomycin Resistance (Van A)
Cutibacterium acnes/Propionibacterium acnes Vancomycin Resistance (Van B)
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium
Escherichia coli
Hemophilus influenzae
Klebsiella (enterobacter) aerogenes
Klebsiella oxytoca
Klebsiella pneumoniae
Moraxella catarrhalis
Moraxella lacunata
Peptostreptococcus spp
Prevotella bivia
Pseudomonas aeruginosa
Serratia marcescens
Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus haemolyticus
Staphylococcus lugdunensis
Staphylococcus saprophyticus
Stenotrophomonas maltophilia
Streptococcus agalactiae (Group-B)
Streptococcus pneumoniae
Streptococcus pyogenes (Group-A)
Vibrio spp/Vibrio vulnificus

Instruction for Use

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Safety Data Sheet

Sensitivity: < 10copies/ul
Run Time: Approx 1:00 hr (depending on the instrument)
Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time PCR platforms.
Catalog# Reactions
Quantity per Kit Description
ENT-4-12-192T 192 12x2 PCR Reagents (750 µL) in tubes (pre-mixed).
ENT-4-12-96P 192 24 Multiplex PCR Reagents pre-plated 96 well breakaway plates. (8 reactions [6 samples] per plate)
ENT-4-12-192P 192 12 Multiplex PCR Reagents pre-plated 384 well HALF (½)plates (16 reactions [14 samples] per plate)
ENT-4-12-384P 192 6 PCR Reagents pre-plated 384 well plates (32 reactions [30 samples] per plate)

For Research Use Only

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