This respiratory pathogen panel (RPP) polymerase chain reaction (PCR) pathogen panel is intended for qualitatively, or semi-quantitatively, detecting the infectious pathogens causing respiratory tract infection and the antibiotic-resistant genes associated with the pathogens. This Scienetix molecular RPP kit contains qPCR multiplex reaction mixes for detecting twenty-one (21) viral and fourteen (14) bacterial pathogens. The Scienetix kit also includes two (2) assays for detecting antibiotic resistance genes and one (1) amplification control. This RPP kit includes ten (10) validated multiplex reaction mixes (RRM-1 to RRM-10) and all associated qPCR assay controls. The RPP kit detection reagents are manufactured and packaged as an open system reagent (OSR) for use with open system platforms. Examples of open system platforms are the Applied Biosystems QuantStudio (Design & Analysis software version 1.5.1 or later), Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time qPCR platforms.
This RPP qPCR reagent kit consists of pre-formulated qPCR reactions and standards for monitoring assay performance. Scienetix reagents are available in a pre-mixed reaction mix provided in 2mL tubes or pre-plated in 96 and 384 well plates. It is recommended to test amplification controls (AC) and negative controls (NC) with each lot (qPCR plate) of sample testing.
| Virus (21) | Bacteria (14) |
|---|---|
| Human coronavirus 229E | B. parapertussis |
| Human coronavirus HKU1 | Bordetella Pertussis & Holmesii |
| Human coronavirus NL63 | Chlamydophila pneumoniae |
| Human coronavirus OC43 | Klebsiella pneumoniae |
| Human gammaherpesvirus 4 (Epstin-Barr, Mono) | Moraxella catarrhalis |
| Human parechovirus | Staphylococcus aureus |
| Human bocavirus | Haemophilus influenzae |
| Human adenovirus | Haemophilus influenzae B |
| Human parainfluenza 1 virus | Salmonella typhi/paratyphi |
| Human parainfluenza 2 virus | Mycoplasma pneumoniae |
| Human parainfluenza 3 virus | Streptococcus pyogenes (GAS) |
| Human parainfluenza 4 virus | Legionella sp |
| Influenza A virus H1N1 swl | Streptococcus agalactiae (GBS) |
| Human respiratory syncytial viruses | Streptococcus pneumoniae |
| Human rhinovirus | |
| Influenza A virus | |
| Influenza B virus | AMR (2) |
| Influenza c virus | Van A/B |
| EV-A71 | Methicillin Antibiotic resistance |
| Human metapneumoviruses | |
| EV-D68 |
| Catalog# | Reactions(10µl) | Quantity per Kit | Description |
|---|---|---|---|
| RPP40-96PT | 96 | 12 | RPP Multiplex qPCR Reagents pre-plated 96 well plates(8 samples per plate) |
| RPP40-384PT | 96 | 3 | RPP Multiplex qPCR Reagents pre-plated 384 well plates(32 samples per plate) |
| RPP40-96 | 96 | 10 | RPP Multiplex qPCR Reagents pre-mixed tubes RRM 1-10 |
For Research Use Only
