Respiratory Pathogen Panel (RPP)
PCR Reagents Kit

This respiratory pathogen panel (RPP) polymerase chain reaction (PCR) pathogen panel is intended for qualitatively, or semi-quantitatively, detecting the infectious pathogens causing respiratory tract infection and the antibiotic-resistant genes associated with the pathogens. This Scienetix molecular RPP kit contains qPCR multiplex reaction mixes for detecting twenty-one (21) viral and fourteen (14) bacterial pathogens. The Scienetix kit also includes two (2) assays for detecting antibiotic resistance genes and one (1) amplification control. This RPP kit includes ten (10) validated multiplex reaction mixes (RRM-1 to RRM-10) and all associated qPCR assay controls. The RPP kit detection reagents are manufactured and packaged as an open system reagent (OSR) for use with open system platforms. Examples of open system platforms are the Applied Biosystems QuantStudio (Design & Analysis software version 1.5.1 or later), Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time qPCR platforms.

This RPP qPCR reagent kit consists of pre-formulated qPCR reactions and standards for monitoring assay performance.

  • Reaction Mix (RRM-1 to 10)
  • Positive Control (PC)
  • Exogenous Extraction + RT Control (EERTC)
Virus Bacteria AMR
Enterovirus-A71 Bordetella parapertussis Methicillin Resistance (MecA)
Enterovirus-D68 Bordetella pertussis & holmesii Vancomycin Resistance (VanA)
Human coronavirus 229E Chlamydophila pneumoniae Vancomycin Resistance (VanB)
Human coronavirus HKU1 Haemophilus influenzae
Human coronavirus NL63 Haemophilus influenzae B
Human coronavirus OC43 Klebsiella pneumoniae
Human Gama 4 (Epstein-Barr, Mono) Legionella spp.
Human adenovirus Moraxella catarrhalis
Human bocavirus Mycoplasma pneumoniae
Human parechovirus Salmonella typhi/paratyphi
Human parainfluenza 1 virus Staphylococcus aureus
Human parainfluenza 2 virus Streptococcus agalactiae (GBS)
Human parainfluenza 3 virus Streptococcus pneumoniae
Human parainfluenza 4 virus Streptococcus pyogenes (GAS)
Influenza A virus H1N1 swl
Human respiratory syncytial viruses (A/B)
Human metapneumoviruses
Human rhinovirus
Influenza A virus
Influenza B virus

Instruction for Use

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Safety Data Sheet

Sensitivity: < 10copies/ul
Run Time: Approx 1:00 hr (depending on the instrument)
Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time PCR platforms.
Catalog# Reactions
Quantity per Kit Description
RPP-4-10-192T 192 10x12 Multiplex PCR Reagents pre-mixed tubes
RPP-4-10-96P 192 24 Multiplex PCR Reagents pre-plated 96 well break away plates (8 reactions [6] samples] per plate)
RPP-4-10-192P 192 12 Multiplex PCR Reagents pre-plated 384 well HALF plates (16 reactions [14 samples] per plate)
RPP-4-10-384P 192 6 Multiplex PCR Reagents pre-plated 384 well plates (32 reactions [30 samples] per plate)

For Research Use Only

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