This nail pathogen (NP) polymerase chain reaction (PCR) pathogen panel is intended for qualitatively, or semi-quantitatively, detecting the infectious pathogens causing nail infection and the antibiotic-resistant genes associated with the pathogens. This Scienetix NP kit contains multiplex qPCR assays for detecting ten (10) bacteria and five (5) fungal pathogens. The Scienetix kit also includes three (3) assays for detecting antibiotic resistance genes and one (1) internal control. This NP kit includes six (6) validated multiplex reaction mixes (NPRM-1 to NPRM-6) and all associated qPCR assay controls. The kit detection reagents are manufactured and packaged as an open system reagent (OSR) for use with open system platforms. Examples of open system platforms are the Applied Biosystems QuantStudio (Design & Analysis software version 1.5.1 or later), Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later), Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time qPCR platforms.
This NP reagent kit consists of pre-formulated qPCR reactions and standards for monitoring the assay performance. Scienetix reagents are available in a pre-mixed reaction mix provided in 2mL tubes or pre-plated in 96 and 384 well plates. It is recommended to test amplification controls (AC) and negative controls (NC) with each lot (qPCR plate) of sample testing.
| REAGENT | QTY | STORAGE |
|---|---|---|
| Nail Pathogen PCR Reaction Mix Tubes (NPRM-1 to 6) | 1 | -20°C |
| Amplification Control (AC) – 200µL | 1 | -20°C |
| Catalog# | Reactions(10µl) | Quantity of Plates | Description |
|---|---|---|---|
| NP-96PT | 96 | 6 | Nail Pathogen Multiplex qPCR Reagents pre-plated 96 well plates (16 samples per plate) |
| NP-384PT | 192 | 3 | Nail Pathogen Multiplex qPCR Reagents pre-plated 384 well plates (64 samples per plate) |
| NP-96 | 96 | 1 | Nail Pathogen Multiplex qPCR Reagents pre-mixed tubes NPRM 1-6 |
For Research Use Only
